Mitochondrial DNA(mt DNA) of Mammalian is the circular one which the 16.5K base pairs md show the maternal inheritance.
Evolutional speed of nucleotide sequence is very fast. So that polymorphic analysis of mt DNA provide the useful informations to investigate the genetic relations of interspecies.
Authors trials were focussed to compare with the polymorphic differences of mitochondrial DNA between Jindo and Japanese mongrel dogs.
DNA was extracted from bloods of 21 head of Jindo dogs and 20 head of Japanese dogs and isolated using 10 kinds of restriction endonucleases(Apa I , BamH I , Bgl II, EcoR I ,EcoR V, Hinc II. Hind III, Pst I , Sty I , Xba I) and then separated by the agarose gel electrophoresis.
After sourthern blotting hybridization was completed using the mtDNA of Japanese mongrel dogs as a probe.
Autoradiography was used to compare the polymorphism of mtDNA both dogs.
The results obtained were as follows
1. mt DNA of Jindo dog showed polymorphism resulting cleavage with four kinds of restriction endonuclease, Apa I , EcoR V, Hinc II, Sty I. While in the Japanese mongrel dogs observed the polymorphism in the five kinds of restriction endonuclease supplemented with EcoR I .
2. Compared with both dogs the frequency differences of DNA polymorphism were recognized in the specific restriction endonuclease Apa I. Consequently in the restriction endonuclease APa I both dogs classified with three types as A, B, C however in the Jindo dogs frequency of C type was 71.5 percent but in Japanese mongrel dogs observed 45 percent in the A type.
3. DNA polymorphism obtained from the use of five kinds of restriction endonuclease were classified with seven types.
In Jindo dogs frequency was highest in the type 6 as 71.4 percent but in the Japanese mongrel dogs showed 35 percent in the type 5.
4. Genetic distances calculated by NEI method showed 0.0089 in Jindo dogs and was 0.0094 in the Japanese mongrel dogs.
|